Shailin FIRST Member Star(s) Indication of membership status - One star is a FIRST member, two stars is Double Gold Scientist, 24/7 Student

29 years old
Not Specified
from Irvine, CA

  • Activity

    • Sometimes dreams can make us feel lost

      1 year ago

      Shailin Scientist, 24/7 Student

      There are lots of dreams we remember and also those we forget. I think of it as my brain is just running through every thought in my mind and fleshing them out, almost like a series of simulations. Some are good, bad, scary, and wonderful. I say wonderful compare to good because sometimes you feel like a dream crosses over the space of reality. Idk not to get too crazy about it, but sometimes its nice to process a dream after you wake up. This morning i had a dream that was wonderful but it left me feeling lost this morning. Lost in the sense that it made me realize some things that were missing from my life or at the very least are attainable if I try to do things differently. Lately I have been feeling like there needs to be some changes for the better in my life and I think inadvertently this is my way of processing those thoughts. 


      Lately i feel like, there is a lot that I can do to improve and enjoy my life more, but it in some ways overwhelms me with the myriad of things to do. I want to take things one at a time, because I just want to move forward. Wow that was vague, anyway what i just wanted to share is that I want to move forward and I just need to learn to do it in a way that doesn't overwhelm me. 


      I feel a small sense of catharsis wrtiing this, I dont know how often i will do this but i want to try and put my emotions to words in a place that seems normal and relaxing, i always forget this place was also a social site for the community 


      -ttyl

    • Macgyvered an RNA Extraction Recovery / Got to Rank 20 with Yogg Hunter

      3 years ago

      Shailin Scientist, 24/7 Student

      So this week was lose lose then win win kinda of week.


      Basically I was gearing up to setup an extraction of RNA from fresh tumor samples onto a robot that we use for automation.... Guess I should give some context, I work in a research lab and some of my best moments at work involve using cube shaped robots that take care of the bulk of the work and I love them fore that so I can focus on other things. Anyway it just so happens that this week the robot "decided" to crash leading to the possibility of losing the ability to get RNA from these samples. I was absolutely peeved out my mind because I was not able to recover the extraction using the robot. I have worked with robotics platforms before and usually there is a way to access the logs and backtrack where the problem occurred, however on this particular robot, everything was password protected and shrouded in secrecy and since I am have no time to figure out how to crack 128-bit encryption at this time I felt I lost this cause. But then I figured, OK I have a "relatively" working understanding of science, surely I can figure out something to keep the extraction going, so I started pulling out every and buffer solution I had from other kits to see if I could do this myself. So i made a lot of "educated" assumptions based on other extraction protocols to come up with the best alternative and finish the extraction manually.


      You see nucleic acids like DNA and RNA can be extracted using the process of precipitation. All you really need is a detergent to break open and lyse the cells and then a solution to precipitate the nucleic acid and then wash it to purify it. There is actually a straight forward way to extract DNA from strawberry seeds if anyone wants to try it:

      1. Place 5 star berries in a zip lock bag and completely mash them as much as you can without tearing the bag.

      2. Then fill the bag with about and equal volume of dish soap as the volume of mushed strawberries. The dish soap helps to break up the cells which are the seeds of the strawberries (since fruits are mature ovaries of flowers and because strawberries are aggregates they have a lot of seeds to work with)

      3. At this point take that slurry of soap and strawberries and place it on a coffee filter op top of a cup. The "lysate" or slurry of broke up cells containing DNA will be filtered allowing most of the DNA to be in the cup and the rest of the debris to be removed.

      4. Then add rubbing alcohol, 91% or better so that it is an equal volume to the filtered liquid in the cup. You should now start to see the DNA precipitate between the two layers (top layer is alcohol, bottom layer is filtered liquid) You will see stringy like molecules, that is your strawberry DNA. Simply take a fork or a Popsicle stick with some notches and you can twirl the DNA out of there.

      5. You can take the DNA out and then potentially wash it with more alcohol using a coffee filter again.


      Anyway I explain this because basically my RNA samples were stuck at the "lysate" step and I had no way to clean my RNA so I could finish the extraction, that's where the robot stopped. So we decided to use the closest buffer we had that was used for a similar type of reaction and then tried it with this extraction. And we had a bittersweet successful recovery of samples. I say bittersweet because although I had RNA, it wasn't the cleanest thing in the world since I didn't use the proprietary buffers, but you know what it got the job done. I thought it wasn't gonna be possible to recover it in a meaningful way but I did it anyway and it was a success. One of the first things that came up in my head was when Gus said he would go to Mars, "out of spite mother fucker".


      I felt that exact way when the company who made the robot said I couldn't recover the sample. I did it for the right reasons, but as an after thought it really was "out of spite mother fucker!" -GUS SOROLA.


      Anyway part two... haha I felt so good after sorting that out that I decided to jump onto to Hearthstone after so long to relax.


      I returned to the game fresh into the Old gods meta. After opening a new pack of cards, I got this strange card called Yogg-Sarron, If you are playing this current meta you may know exactly what card I am talking about. So I normally play "face hunter" I know that seems to be a long ago strategy in the current meta, seems like it got a bad rap for sounding like an easy route for playing in Hearthstone. I originally got into it accidentally because I just loved summoning all the cool beast cards back in the old days, it jsut so happens that it gives me plenty of opportunities to attack face. Anyway so I use a lot of spells anyway so I decided to add Yogg to my deck because it has an effect to "cast a random spell for each spell i have played this game" so I play a couple rounds and I finally make it to rank 24. I usually am a perpetual angry chicken during the seasons. It helps now that we do not lose ranks until 20. So I am slowly making my way up to Rank 21 but I have yet to draw Yogg and play it. I was beginning to think I never added it to my deck. So on the last game I played before I reached Rank 20, I finally got to play Yogg. Now mind you this was after playing 16 spells. Yes you heard me right 16 spells because the opponent gave me some spells, well bananas because of Mukla. anyway so I have four hounds on the board and they have one Fog Creeper with taunt, they have 20 blood and I have 14 blood. I drop Yogg and the ensuing chaos that took place was astounding.


      Oh my god first all my house gained +3/+3 somehow and I gained 10 armor. Then lose 10 blood and my opponent gained 6 blood. my whole board got wiped out and then so did theirs. But even though Yogg was dead it kept going. I then gained 5 new minions with a combined power of 32 blood and then my hero power changed to a priest and I healed myself. And then finally I was able to summon all my hunter companions which I immediately found and crafted after this game was done. At this point I was so shocked at the turn of events and in two turns I wiped them out. I finally reach rank 20 which I have never been able to do since the start of Hearthstone. This was a weird week, but it was such a memorable one and I am ended up positive by the end of it so I am definitely happy. Definitely gonna work on my hunter deck this weekend. If anyone knows of some good tips for Hunter in the current meta that would be most appreciated.


      Thanks for listening

      -Shailin

    • Experience at Anime Expo 2016, GOOD and now it makes me want to go to RTX even more.

      3 years ago

      Shailin Scientist, 24/7 Student

      Hi,

      I guess this will be my first "journal" like entry on the site.

      Basically I wanted to recap what happened during my AX trip this year and share my thoughts and open up to the RT community more.


      I had a great experience this year, I honestly think this year was my favoirte so far. I have been going to AX since 2014 and before this year I can summarize it in one phrase: waiting in line and buying things.


      Now that doesn't carry a negative connotation when I say it, its just that the last two times I was so gung ho about going to panels and since the con is so huge, jsut to see those panels takes up a good chunk of the day. but thanks to it I was able to briefly see and talk to Goku (Sean Schimel) and see the Little Witch Academia Ep 2 premiere, which by the way was amazing and so worth it to see because I love studying and learning about new animation projects.


      Anyway but this year because one I worked basically 16 hours straight the day before the weekend started in my lab, I was tired to point of hallucinations haha. Don;t worry I got plenty of sleep this weekend and relaxed at AX. Though seriously I think Ashley Jenkins has the right idea keeping a bed at work, It would be so helpful having that between experiments I run. Speaking of which loved the RT podcast with the Evo Biologist, I do gene sequencing myself so I always dream of working on a gene sequencing project for determining evolutionary lines.


      Anyway my goodness tangent after tangent... because I was so tired this week. I decided with my friends to just go to AX on Sunday and not plan to particularly wait for any panel, just walk around and see whats in the exhibit hall and artist's alley and maybe go to some less busy panels we like.


      Was not expecting to see such amazing people out on the floor. Martin aka Little Kuriboh who does the Yugioh Abridged series, who was just a lovely person to talk with, and Lanipator and Takahata 101 of Team Four Star who do DBZ abridged. Not to mention Aisha, the Yellow Power Ranger was there and walking through were Noble Senpai, Misty Cronexia, Akidearest, and the Anime Man; all amazing anime youtubers that I watch on a regular basis. My mind was just blown. I always figured because I never went to Anime Expo in it's "younger" years that I would never be able to experience the direct interaction between content creators and fans since now there are just so many awesome people who loves these things that creators and talent just can't spend as much time as they want with every fan, which I already made my piece with because I am empathetic to their feelings.


      It;s kinda like how Michael Jones put it in the OFF Topic Podcast last week, there are jsut so many people that one to one conversations just aren't always likely to happen and they feel bad and don't want to give a fan a bad experience.


      I have never really met or interacted with the people I am fans with and to be honest I get too shy and it takes every once of my strength just to come up to someone like them and say hi. When I say Aisha the yellow ranger I was scared at first and just went up and told her how much I could believe that I was seeing her here and briefly talked about the power rangers movie she did. She was super nice and I walked away happy.


      When I saw Little Kuriboh and Team Four Star had set up booths together, I almost lost it, I was squealing in my mind so loud I was about to faint. I went to Little Kuriboh first, I actually had a lovely conversation with Martin about the latest episode. He was so firendly and he just loved having conversations with fans. We actually had a really nice talk getting to know eachother. I opened up to him and told him about how when I lost my mom back in college and just whenever I went through horribly depressive times in my life, how his style of comedy and Team Four Stars where what I relied on to cheer me up. Plus, it's Yugioh abridged and DBZ abridged along with others that I listen to at work when my research stresses me out. I started to tear up a little finally getting to tell Little Kuriboh about my appreciation for the show and he was so nice to me and i took a great photo with him. For a split second I just felt so elated and felt like i finally got the con experience I always dreamed about. I know getting a one on one conversation won't always happen when I go to conventions, but it was a great experience nonetheless. I then was so exhausted, mostly because I was so shy trying to talk to Little Kuriboh, and talking about my mom's death always leaves me temporarily a little dead inside.


      I went over to the Team Four Star booth after and becasue they were so busy with selling and autographing tshirts, I was reluctant to go up because I was afraid I might take time away from other fans, I know I just get too self conscious about these things. It wasn't until my friend pushed me forward up them that I went to say hi, seriously thank Kaveh for doing that. Anyway I got to talk to Lani for bit and he found me two shirts that were in my size and I loved that I got to talk to Takahata about the song he did for Perfect cell. If you haven't heard it is the beautiful song. I listen to it all the time as a work playlist. Takahata and Lani were so friendly and they sign some shirts I got and I was again so happy.


      I guess the reason I am ultimately writing about this is that I finally felt like I experienced this Anime convention as "a part of the anime community". It sounds silly but this year really felt like I went because I wanted to be a part of the community and not just go because there was cool stuff to buy. Maybe because it was no longer my first time here we took a more relaxed approach to it so I could just take it all in more. Before I just felt like running from panel line to panel line in search of something to do instead of just being at the convention. There is no one right way to experience a convention, so there wasnt anything wrong with my previous experiences at Anime Expo it's just that I felt more at home, which I wasn't expecting since it is such a large convention.


      More to the point, I really want to go to RTX next year. Again I wish I could have gone to the first one and visit each year because I yearn for this sense of community that exists within fandom. I guess that's why I want to start posting journals on RT. I love the RT community, it has always seemed so nice and friendly and open. I feel like it's something that isn't grandiose that feel line a small fish in a sea. Not to say it isn't large, it's just that it seems less intimidating to get into. I want to try and make more interactions here. Even if no one reads this post, I just want to start putting myself out here more because I want to interact with more people, maybe this will also help with my shyness too. Hopefully in the next year, I make more friends here on Roosterteeth, so that when I go to RTX, I won't feel like a small fish not sure where to swim. I am also glad I have one of my close friends that will maybe go with me too. But it was something that I think Jack Patillo said about RTX that really got to me that made me want to go, when he said that when you go to RTX try to make new friends because you are in a place where everyone here likes at least one thing I do. Making new friends and being a part of the RT community would bring me absolute joy. So thanks to my amazing time at Anime Expo this year and some great words from last weeks Off Topic podcast from Michael, Jack, Lindsay, and Jeremy, I really plan on going to RTX 2017 and will try to interact with the RT community more. Also PS I will make sure my camera is always ready before I go up to someone I want to take a picture with, of course I will need to work on first getting more courage to go up to someone of RT that I want to talk to but, you know it's progress.


      If anyone reads this and would maybe wanna talk hit me up, because it would be a pleasure to meet you.

    • 3 years ago

      Shailin Scientist, 24/7 Student
    • RWBY game so excited!!

      3 years ago

      Shailin Scientist, 24/7 Student

      I am so excited for the RWBY game! I love Hack n Slash games! A good chunk of steam collection is mostly HNS games. Not to familiar with the MOBA style of leveling but I am starting to get into Heroes of the storm and so far I really like playing as Tassadar, mind you it's cause I shelled out for the Transformer Skin because why not.

      Anyway I cant wait till the game is released.

    • 3 years ago

      Shailin Scientist, 24/7 Student
    • 2019 years ago

      Shailin Scientist, 24/7 Student
  • Comments (2)

    • Tom0

      4 years ago

      Welcome to the RT community :)

      • Shailin FIRST Member Star(s) Indication of membership status - One star is a FIRST member, two stars is Double Gold Scientist, 24/7 Student

        4 years ago

        Thanks nice to meet you :)

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    Awesome Team Four Star shirts from Lani and Taka

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